Fig. 2.

Changes of urinary protein excretion (UP), glomerulosclerosis (GS), and expression of each of the proteins in PECs in the rat model of adriamycin (ADR) nephropathy. Rats were injected with ADR, and urinary protein excretion and kidney sections were examined on days (d)0, 8, 14, and 42 (n = 6 at each time point). A: urinary protein excretion. B: glomerulosclerosis. C: sestrin 2. D: α-smooth muscle actin (α-SMA). E: phosphorylated S6 ribosomal protein (P-S6RP). F: CD44. G: paired box 2 (PAX2). The degree of GS (B) was estimated on periodic acid-Schiff (PAS)-stained kidney sections as described in methods. Degrees of sestrin 2 (C), α-SMA (D), P-S6RP (E), CD44 (F), and PAX2 (G) expression were estimated on immunohistochemically stained kidney sections using each specific antibody as described in methods. *P < 0.01 vs. day 0; #P < 0.01 vs. day 8; ##P < 0.05 vs. day 8; §P < 0.01 vs. day 14.