Fig. 5.

Changes of urinary protein excretion, glomerulosclerosis, and expression of each of the proteins in PECs of the rat model of puromycin aminonucleoside (PAN) nephropathy. Rats were injected with PAN, and urinary protein excretion and kidney sections were examined on days 0, 9, and 28 (n = 6 at each time point). A: urinary protein excretion. B: glomerulosclerosis. C: sestrin 2. D: α-SMA. E: P-S6RP. F: CD44. G: PAX2. The degree of glomerulosclerosis (B) was estimated on PAS-stained kidney sections as described in methods. Degrees of sestrin 2 (C), α-SMA (D), P-S6RP (E), CD44 (F), and PAX2 (G) expression were estimated on immunohistochemically stained kidney sections using each specific antibody as described in methods. *P < 0.01 vs. day 0; **P < 0.05 vs. day 0; #P < 0.01 vs. day 9.