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. 2014 Jul 16;307(6):C542–C553. doi: 10.1152/ajpcell.00033.2014

Fig. 1.

Fig. 1.

Overexpression of RhoA in the partial bladder outlet obstruction (pBOO) murine model. RT-PCR (A and B) and immunoblot (C and D) analysis of RhoA mRNA and protein expression in sham-operated and pBOO mouse detrusor smooth muscle (DSM) tissues. GAPDH served as a loading control. B and D: quantification of RT-PCR (A) and immunoblot (C) data presented as the means ± SD (n = 10; 10 sham-operated and 10 pBOO mice DSM were used in this experiment). **P < 0.01 compared with the results for sham-operated control mice. E: murine bladder sections prepared from sham-operated and pBOO mice were stained with anti-RhoA (Cy3, red) and anti-SM22 (FITC, green) antibodies. Scale bars = 20 μm.