Fig. 3.

RhoA overexpression in DSM from human bladders with benign prostatic hyperplasia (BPH)-induced bladder outlet obstruction. A–D: RNA and protein samples prepared from DSM of control and patients with BPH were subjected to RT-PCR and immunoblot analysis for RhoA. GAPDH was used as a loading control. B and D: quantification of RT-PCR (A) and immunoblot (C). Data are presented as the means ± SD (n = 6; 6 normal and 6 obstructed human bladders were used in this experiment). **P < 0.01 compared with the results for control (normal) human DSM. E: human bladder sections prepared from control and patients with BPH were stained with anti-RhoA (Cy3, red) and anti-smooth muscle myosin heavy chain (SMHC) (FITC, green) antibodies. Scale bars = 20 μm.