Fig. 4.

ROCK-β overexpression in DSM from human bladders with BPH-induced bladder outlet obstruction. A–D: RNA and protein samples prepared from DSM of control and patients with BPH were subjected to RT-PCR and immunoblot analysis for ROCK-β. GAPDH was used as a loading control. B and D: quantification of RT-PCR (A) and immunoblot (C). Data are presented as the means ± SD (n = 6; 6 normal and 6 obstructed human bladders were used in this experiment). **P < 0.01 compared with the results for control human DSM. E: human bladder sections prepared from control and patients with BPH were stained with anti-ROCK-β (Cy3, red) and anti-SMHC (FITC, green) antibodies. Scale bars = 20 μm.