Fig. 7.

Stretch upregulates NF-κB and GATA-6 DNA binding activity. A and B: nuclear extracts from human BSM cells exposed to stretch or control were assayed using a transcription factor ELISA to determine DNA-binding activity of GATA-6 and NF-κB. The graph shows binding of GATA-6 (A) and NF-κB p50 (B) to their consensus oligonucleotides expressed as absorbance units at 450 nm. In oligonucleotide competition assay, increasing amounts of wild-type (WT) or mutant NF-κB-binding and WT or mutant GATA-6-binding consensus DNA sequence were added to the ELISA plate. Data are representative of at least 3 independent triplicate experiments. **P < 0.01 compared with control cells.