Fig. 8.

Stretch upregulates NF-κB and GATA-6 DNA binding to CPI-17 promoter. A–F: chromatin samples prepared from control (marked as C) and stretched DSM cells were immunoprecipitated with anti-GATA-6, anti-NF-κB p50 anti-c-Rel, and anti-upstream stimulatory factor (USF)-1 antibodies. The anti-USF-1 antibody served as a negative control. Precipitated fragments were PCR amplified using primers specific for the GATA and κB motifs on the mouse CPI-17 promoter (top) or CPI-17 exon-1 (bottom). B, D and F: quantification of band intensities from agarose gels (A, C, and E) are presented as a percentage of input chromatin. Data are presented as the means ± SD (n = 6; 6 of control and 6 of stretched cell samples from separate experiments were used in this experiment). **P < 0.01 compared with control cells.