Skip to main content
. 2014 Jul 16;307(6):C542–C553. doi: 10.1152/ajpcell.00033.2014

Fig. 9.

Fig. 9.

Stretch promotes the transcriptional activation of CPI-17 promoter by GATA-6 and NF-κB in primary BSM cells. A: human BSM cells were cotransfected with murine WT CPI-17 promoter luciferase constructs and GATA-6, NF-κB p50, and c-Rel cDNA. Luciferase activity was measured in each sample after 72 h and is presented relative to that of Renilla luciferase. Data are presented as means ± SD from 5 independent experiments. **P < 0.01 compared with stretched cells. B: human BSM cells were transfected with murine WT or mutant CPI-17 promoter luciferase constructs. The NF-κB binding site (κB motif) mutation in the 1.33-kb CPI-17 promoter is designated MUT1. The GATA-6 binding site (multiple GATA motifs) mutation is designated MUT2. The double mutant of both Mut1 and Mut2 mutations is designated MUT3. Luciferase activity was measured in each sample after 72 h and is presented relative to that of Renilla luciferase. Data are presented as means ± SD from 5 independent experiments. **P < 0.01 compared with stretched cells.