Fig. 1.
Representative digital images of the subfornical organ (SFO) expressing green fluorescent protein from a rat injected with the viral transduction construct containing either adeno-associated viral-green fluorescent protein-scrambled AAV-short hairpin RNA (AAV-GFP-SCM shRNA) (A) or shRNA against AT1a receptor (AAV-GFP-AT1a shRNA) (B) into the SFO. Scale bar is 50 μm. RT-quantitative PCR analysis (C) of AT1aR mRNA from a laser-microdissected SFO nuclei from sham and BDL rats injected with either a scrambled AAV-shRNA (SCM) or an AAV-shRNA targeted to AT1a gene (sh-AT1a) into the SFO. The data are presented as means ± SE relative mRNA levels as calculated by the 2−ΔΔCT method. *Statistically significant, using ANOVA with Student-Newman-Keuls (SNK) post hoc test. P < 0.02 vs. all other groups; n = 5 for each group.