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Relaxation of fetal abdominal aortas in response to increasing doses of ACh. A–D: arteries were precontracted with EC80 dose of U46619. ACh dose-response curves were compared using two-way ANOVA followed by a Bonferroni post hoc test where appropriate. All data are expressed as means ± SE (n = number of animals). ■ denotes WT male, while ● denotes WT female, □ denotes P0 male, and ○ denotes P0 females. Asterisks denote level of significance using Bonferroni post hoc test. *P < 0.05.
