Fig. 2.

Analysis of expression of Bruton's tyrosine kinase (Btk; A) and MyD88 (B) in mouse alveolar neutrophils purified from mice treated with LPS and ICs (LPS/IC), and mice treated with LPS and Saline (LPS/Sal). Vertical bar charts in A and B depict the fold increase in the levels (fluorescence intensity) of Btk and MyD88 in lung neutrophils from LPS/IC mice compared with LPS/Sal mice. C: histogram presenting colocalization between Btk and MyD88 in lung neutrophils of LPS/IC and LPS/Sal mice. Vertical bar chart in C shows correlation factor (Correlation R) indicating the level of colocalization between Btk and MyD88 in lung neutrophils of LPS/IC and LPS/Sal mice. D: analysis of levels of phosphorylated Btk (pBtk) in mouse alveolar neutrophils purified from either mice treated with LPS and ICs (LPS/IC) or animals treated with LPS and Saline (LPS/Sal). The vertical bar chart in D depicts the fold increase in the level (fluorescence intensity) of pBtk in lung neutrophils from LPS/IC compared with LPS/Sal mice. Alveolar neutrophils from 3 animals per group were analyzed (160–250 cells were scanned), and typical findings are presented. In A–D samples were collected at 2 h after anti-KC:KC IC administration. A.U., arbitrary units.