Fig 6.

Treatments with 1% or 2% isoflurane for 1, 3, and 6 h on caspase-3 activation in primary neurones of mice. Treatment with 2% isoflurane for 1 h does not induce caspase-3 activation in the neurones (a and b). The treatments with 1% isoflurane for 1 (c and d), 3 (e and f), and 6 (g and h) h do not induce caspase-3 activation in the neurones. The treatments with 2% isoflurane for 1 h (a and b), 1% isoflurane for 3 h (e and f), and 1% isoflurane for 6 h (g and h) decrease the caspase-3 activation.