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. 2014 Apr 3;113(4):695–707. doi: 10.1093/bja/aeu053

Fig 7.

Fig 7

Dantrolene attenuates the isoflurane-induced ER stress and caspase-3 activation in the primary neurones. (a) Treatment with 2% isoflurane plus DMSO for 6 h (lane 3) increases CHOP levels when compared with the control plus DMSO condition (lane 1) in the primary neurones. Treatment with isoflurane plus dantrolene (lane 4) leads to a reduction in the CHOP levels when compared with the treatment with isoflurane plus DMSO (lane 3). There is no significant difference in amounts of β-actin among the different groups. (b) Quantification of the western blot shows that isoflurane plus DMSO treatment (pink striped bar) increases CHOP levels when compared with the control plus DMSO condition (blue bar), normalized to β-actin levels, whereas dantrolene (orange striped bar) attenuates the isoflurane-induced increases in the CHOP levels, normalized to β-actin levels. (c) Treatment with 2% isoflurane plus DMSO for 6 h (lanes 7–9) increases the cleaved caspase-12 levels when compared with the control plus DMSO condition (lanes 1–3) in the primary neurones. Treatment with isoflurane plus dantrolene (lanes 10–12) leads to a lesser degree of cleaved caspase-12 levels compared with the treatment with isoflurane plus DMSO (lanes 7–9). There is no significant difference in amounts of β-actin among different groups. (d) Quantification of the western blot shows that isoflurane plus DMSO treatment (pink striped bar) increases the cleaved caspase-12 levels compared with the control plus DMSO condition (blue bar), normalized to β-actin levels, whereas dantrolene (orange striped bar) attenuates the activation of caspase-12 induced by isoflurane plus DMSO (pink striped bar), normalized to β-actin levels. (e) Treatment with 2% isoflurane plus DMSO for 6 h (lanes 7–9) induces caspase-3 activation when compared with the control plus DMSO condition (lanes 1–3) in the primary neurones. Treatment with isoflurane plus dantrolene (lanes 10–12) induces a lesser degree of caspase-3 activation when compared with the treatment with isoflurane plus DMSO (lanes 7–9). There is no significant difference in the amounts of β-actin among different groups. (f) Quantification of the western blot shows that isoflurane plus DMSO treatment (pink striped bar) induces caspase-3 activation compared with the control plus DMSO condition (blue bar), normalized to β-actin levels, whereas dantrolene (orange striped bar) attenuates the isoflurane-induced caspase-3 activation, normalized to β-actin levels.