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. 2014 Sep 18;2:71. doi: 10.3389/fchem.2014.00071

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Copyright © 2014 Rodriguez, Papanastasiou, Juba and Bishop.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MALDI-TOF mass spectrum of P4LC (m/z: 2315.5) obtained after purification indicates a 3:1 LVFX/Pep-4 loading stochiometry. No peaks assignable to Pep-4 or intermediately acylated conjugate species were observed. The baseline isotopic distribution for P4LC is shown in the inset with labeling of the monoisotopic peak.