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. 2014 Jul 1;31(10):2573–2585. doi: 10.1093/molbev/msu198

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© The Author 2014. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Caenorhabditis briggsae NURF-1A and ISW-1 control the expression of fog-1 and fog-3. Transcript levels were measured by real-time RT-PCR and calculated using 2exp(−ΔΔC_T), with rpb-1 transcripts as a reference; fog-1 and fog-3 were normalized to wild-type animals at the XX L3/L4 molt and spe-4 to the XX L4. Error bars represent standard error of the mean. (A) Wild-type animals subjected to RNAi. (B) tra-1(nm2) animals subjected to RNAi. P values were determined with a Student’s t-test, one-tailed, with unequal variance. (C) The phenotypes of siblings of animals that were collected for transcript analysis.