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. 2014 Jul 24;31(10):2793–2804. doi: 10.1093/molbev/msu223

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© The Author 2014. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Fig. 5. — Effect of anaerobic conditions and iron chelation on ciprofloxacin-resistant evolution. (A) Effect of anaerobic conditions on the frequency of ciprofloxacin-resistant populations in the case of Δfur, ΔsodAB, and WT strains. Inlet in panel (A): Survival of the WT strain in the presence of ciprofloxacin under anaerobic conditions. Fractions are based on a total of n = 96 populations of each genotypes. (B) Effect of iron chelation using the cell permeable iron chelator o-phenantroline (50 µM) on the frequency of ciprofloxacin-resistant populations. Fractions are based on a total of n = 384 populations of each genotypes. Error bars represent confidence intervals of proportions (Wilson procedure). ***P ≤ 0.001, **P < 0.05 (chi-square test using the actual number of observations). a.c., anaerobic conditions; Phen., o-phenantroline.