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Validation of visualization methods using examples from the X chromosome. See Figure 1 for key. In all panels, female samples are in gray and male samples in green. (A) XIST promoter shows high methylation in males and approximately 50% in females. (B) MAOA, a gene subject to XCI, shows 50% methylation in females and low methylation in males. (C) RPS4X, a gene that escapes XCI, shows low island methylation regardless of sex.
