In this illustrative schematic, proteins B and C work together, and in parallel with
protein A, to carry out function X. Standard E-MAPs exhibit similar genetic profiles for
BΔ and CΔ, but higher order analysis via TMA reveals significant differences
in a AΔ background. Thus, although B and C share an important common function, they
have different secondary functions that were unmasked by TMA. We have used this
methodology to uncover differences between Asf1 and Rtt109. Asf1 is necessary for the
Rtt109-dependent acetylation of histone H3-K56 and their E-MAP profiles are highly
similar. However, TMA in a cac1Δ background revealed significant
differences between asf1Δ cac1Δ and
rtt109Δ cac1Δ in their interactions with
HIRA and SWR-C complex member deletions.