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. Author manuscript; available in PMC: 2015 Jun 15.
Published in final edited form as: Anal Biochem. 2014 Apr 1;455:35–40. doi: 10.1016/j.ab.2014.03.016

Fig. 3.

Fig. 3

Multiplex binary gradient elution profile for quantitation of trimethylamine-N-oxide (TMAO) by LC/MS/MS. Two different solvents, A, 0.1% propanoic acid in water; B, 0.1% acetic acid in methanol, were used to generate the gradient. a1 and a2, time point for sample injection on column 1 and 2 respectively; b1 and b2, time point for start of data collection on column 1 and 2 respectively; c1, c2, time point for data collection to end and column 1 and 2 to be switched to waste, respectively.