Table 4.
Evaluation of three QC plasma TMAO concentrations which were subjected to 5 freeze and thaw cycles.
| QC Plasma |
Plasma TMAO (μM) | CV% | |||||
|---|---|---|---|---|---|---|---|
| Cycle 1 | Cycle 2 | Cycle 3 | Cycle 4 | Cycle 5 | Inter- cycle |
Intra- cycle |
|
| 1 | 1.61 1.68 |
1.77 1.77 |
1.78 1.71 |
1.75 1.74 |
1.71 1.80 |
4.1 |
2.6 |
| 2 | 2.47 2.38 |
2.71 2.60 |
2.99 2.77 |
2.58 2.65 |
2.66 2.58 |
8.8 |
3.3 |
| 3 | 15.2 14.9 |
16.2 15.4 |
16.3 15.2 |
15.1 15.9 |
15.3 15.3 |
2.9 |
3.4 |
Plasma were frozen at −80 °C in dry ice box and thawed to room temperature. The same vial of plasma was subjected to five freeze and thaw cycles. TMAO in each sample at each cycle was measured in duplicate.