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. Author manuscript; available in PMC: 2015 Mar 15.
Published in final edited form as: Cancer Res. 2014 Jul 18;74(18):5019–5031. doi: 10.1158/0008-5472.CAN-13-2845

Fig. 4. The BMPRIA inhibitor DMH1 decreases NK cells cytokine and chemokine production.

Fig. 4

MACS purified NK cells (purity ≥95%) were cultured in IL-2, -12 and poly I:C with or without addition of DMH1 (5 - 50 μM) or DMSO as vehicle control or noggin (100 ng/ml). In (A) p-Smads-1/-5/-8 expression were determined after 20 hours by intracellular staining and flow cytometry. In (B and C) NK cell supernatants were collected after 20 hours of culture and IFN-γ production determined by ELISA. In (D) annexin-V expression was determined by flow cytometry and in (E) the indicated cytokine and chemokine levels were determined using a cytometric bead array system. In (A and D) one representative donor of three is shown and in (B), (C) and (E) the data are the mean ± 1SD of two or three separate donors. *p<0.01 vs. no addition of noggin or DMH1.