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. Author manuscript; available in PMC: 2015 Mar 15.
Published in final edited form as: Cancer Res. 2014 Jul 18;74(18):5019–5031. doi: 10.1158/0008-5472.CAN-13-2845

Fig. 5. DMH1-treated NK cells display a reduced capacity to induce DC maturation.

Fig. 5

NK cells were cultured for 12 hours in culture media or IL-2 (20 ng/ml) or IL-2, -12 and poly I:C with or without addition of DMH1 (20 μM). NK cell were then washed x3 before additional culture alone or together with immature in vitro derived MoDCs at a ratio of 1:5. After 18 hours of culture the levels of CD80 and CD83 expressed by MoDCs (A) was determined by flow cytometry and in (B) the levels of IL-12p70 and TNF-α in the culture supernatants were determined by ELISA. One representative donor of three is shown in (A) and the data are the mean ± 1SD of three separate donors in (B). *p<0.01 vs. IL-2, -12 and poly I:C activated NK cells without DMH1 treatment. In (B) the addition of LPS to MoDC served as a positive control.