Figure 4. Effect of the compromised immune system (nude mice) on systemic oxidative DNA damage and its inhibition by Tempol.
Three types of oxidative lesions, APE1, EndoIII, and hOGG1 (oxidized purines) were measured in normal tissues and tumors of 4 cohorts of mice, control (PBS-injected) and LLC-bearing mice without Tempol treatment and with Tempol treatment. Results are grouped by normal organs examined. Error bars, standard deviations in groups of mice (N=5). Gold asterisks, statistically significant difference between PBS and PBS+Tempol; black asterisks, statistically significant difference between tumors and PBS; blue asterisks, statistically significant difference between tumors and tumors+Tempol; red asterisks, statistically significant difference between tumors and PBS+Tempol. For the PBS+Tempol versus PBS, the reduction of APE1 lesions by Tempol exposure is only significant for stomach and spleen. *** (p<0.001).