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. 2014 Sep 16;9(9):e107734. doi: 10.1371/journal.pone.0107734

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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The left coronary artery was ligated for 30 minutes and then reperfused for 2 hours. Panel A illustrates representative 2,3,5-triphenyltetrazolium chloride (TTC) stained images for each of the four groups of mice. The percent of (B) area at risk over total area (AR/TA), (C) area of necrosis over total area (AN/TA), and (D) area of necrosis over area at risk (AN/AR) were calculated in Nos2+/+ (open bars) or Nos2−/− mice (filled bars) by staining with Evans blue and TTC. Values are mean ±SE. 8–12 mice were studied in each group. *P<0.01 compared with Nos2+/+ mice fed the same diet, #P<0.05 compared with mice of the same genotype fed the control diet by two-way ANOVA.