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. 2014 Sep 16;9(9):e107734. doi: 10.1371/journal.pone.0107734

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Levels of Nos2 mRNA (A) and Nos3 mRNA (B) were measured by real-time PCR in Nos2+/+ (open bars) or Nos2−/− (filled bars) mice. Values were normalized to 18S mRNA and are expressed as percent of the control values observed in Nos2+/+ mice fed the control diet. (C) Tiron-quenchable and NADPH-enhanced lucigenin chemiluminescence was measured in cardiac homogenates. Values are mean ±SE. 6–9 mice were studied in each group. *P<0.05 compared with mice of the same genotype fed the control diet; #P<0.05 compared with Nos2+/+ mice fed the same diet.