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Detection of exogenous pDNA in the mitochondria-enriched fraction before and after DNase treatment. At 24 h after HLV injection of the condensed pDNA, the crural muscles were harvested, and the pDNA in mitochondria-enriched fraction was then measured using q-PCR, before and after treatment with DNase I. Bars indicate means ± S.D. (n = 3). Statistical analysis was performed by a two-tailed unpaired Student’s t-test. N.S. indicates non-significant difference.
