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. Author manuscript; available in PMC: 2015 Sep 15.
Published in final edited form as: Cancer Res. 2014 Jun 13;74(18):5301–5310. doi: 10.1158/0008-5472.CAN-14-0319

Figure 2. GPR81 regulates expression of genes involved in lactate metabolism and lactate uptake and is required for cancer cell survival when lactate is the primary fuel source.

Figure 2

(A-B) Relative mRNA expression of PGC1α, MCT1, MCT4 and CD147 mRNA in BxPC3 (A) or Capan-II (B) si- or shcontrol and si- or shGPR81, grown in DMEM + 10% FBS.

(C) Lactate concentration in the supernatant of Capan-II shControl and shGPR81 were measured using YSI 2900 Biochemistry Analyzer after 24-h of culture in fresh medium; n = 3.

(D & F) Relative mRNA expression of PGC1α, MCT1, MCT4 and CD147 mRNA in (D) Capan-II shControl or shGPR81 cells or (F) ASPC1-low and ASPC1-high cells after lactate stimulation (+) in DMEM without glucose, glutamine or pyruvate + 20mM lactate and 2% FBS for 6 hours normalized to shControl /lactate 0mM. vs. shControl / 20mM lactate. Data are mean ± SEM. n ≥ 3. # vs. lactate 0mM;

(E & G) Lactate concentration in the supernatant of (E) Capan-II shControl or shGPR81 cells or (G) Lactate uptake was measured in ASPC1-low and ASPC1-high cells after 1-h & 6-h of culture DMEM without glucose, glutamine or pyruvate + 20mM lactate and 2% FBS; n = 3.% change lactate uptake was calculated as follows: [lactate-1 hour] / [lactate-6 hour]. **p <0.01 *p<0.05, **p<0.01,***p<0.001 by t-test.

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