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. Author manuscript; available in PMC: 2015 Sep 15.
Published in final edited form as: Cancer Res. 2014 Jun 13;74(18):5301–5310. doi: 10.1158/0008-5472.CAN-14-0319

Figure 3. GPR81 is required for cancer cell survival when lactate is the primary fuel source.

Figure 3

(A-D) Mitochondrial activity of Capan-II shControl and shGPR81 cells (A & B) or ASPC1-low and ASPC1-high cells (C & D) measured by MTS assay in DMEM + 5% FBS (A & C) or DMEM without glucose, glutamine or pyruvate + 20mM lactate and 2%FBS (B & D). Data are mean ± SEM, normalized to day 0. n ≥ 3, . *p<0.05, ***p<0.001; ****p<0.0001 by ANOVA.

(E) Relative cellular death, as measured by Hoescht uptake at 24 hours of shControl and shGPR81 Capan-II cells. Total dead cell area (DAPI stained nuclei)/total cell area (brightfield) was calculated using Simple PCI software and normalized to shControl cells. Data are mean ± SEM, normalized to day 0. n ≥ 3; ****p<0.0001 by t-test.

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