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. 2014 Sep 7;15:105. doi: 10.1186/1471-2202-15-105

Figure 2.

Figure 2

Bar graphs summarizing the percentage distribution of Fluorogold (FG) neurons obtained with the different types of immunolabeling techniques in the ipsilateral and the contralateral ventral tegmental area to the FG injection site in either PL, Cg1-PL or PL-MO sectors of the medial prefrontal cortex. (A) Bar graph showing the percentage distribution of FG-immunolabeled neurons detected with either DAB-immunohistochemistry (IH) or immunofluorescence (IF) methods in the ventral tegmental area ipsilateral and contralateral to the FG injection site in the medial prefrontal cortex. Percentages (mean ± SE) were calculated based on the numbers (961 FG-labeled neurons) obtained from 144 vibratome sections in 18 rats. ANOVA (animal X immunolabeling method X labeling side) was used for statistical comparisons [*p < 0.0001; post hoc Fisher test, factor: labeling side]. (B) Bar graph showing the proportions of single FG- and double FG/tyrosine hydroxylase (TH)-immunolabeled neurons observed in either ipsilateral (ipsi) or contralateral (contra) VTA in tissue double processed for FG and TH. ANOVA (animal X labeling side X single or dual labeling) was used for statistical comparisons [*p < 0.0001; post hoc Fisher test, factor: single or dual labeling]. (C) Bar graph showing the percentage of single FG- and double FG/TH-immunolabeled neurons observed in the three injection groups. Cg1, anterior cingulate cortex; MO, medial orbital cortex; PL, prelimbic cortex. ANOVA (animal X injection site X single or dual labeling) was used for statistical comparisons [*p < 0.0001; post hoc Fisher test, factor: single or dual labeling]. In B and C percentages (mean ± SE) were calculated based on the numbers (466 FG-labeled neurons) obtained from 90 vibratome sections in 18 rats.