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. 2014 Sep 7;15:105. doi: 10.1186/1471-2202-15-105

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© Del Cid-Pellitero and Garzón; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Confocal photomicrographs showing Fluorogold (FG)- and/or tyrosine hydroxylase (TH)-labeled neurons in the ventral tegmental area (VTA) of an animal with an FG injection in the prelimbic cortex. (A-C) , Biomappings showing green FG-labeling (A), red TH-labeling (B) and both (FG/TH) labelings (orange) in the merged image (C). (D-F) , High power confocal photomicrographs of the boxed area in A corresponding to the VTA ipsilateral to the FG injection, depicting FG (D), TH (E) and their merged images (F). Arrowhead marks one VTA neuron dually labeled for FG and TH. Scale bar, A-C, 400 μm; D-F, 40 μm.