Figure 3.

(A) Surface pressure measurements of TM31C in PC/Chol monolayers. The increase in surface pressure (●) is shown after insertion of TM31C into lipid monolayers (π₀ = 24 ± 0.5 mN/m) made of PC and increasing concentrations of Chol; the calculated slopes from the time traces are represented by ○, dotted line. Experimental data are fitted to a polynomial function (degree = 2). Symbols correspond to the mean ± SDs of four to six independent measurements. The mean molecular areas (○, dashed line) were taken from Li et al. (29) to show the increased monolayer packing. (B and C) ANTS-DPX vesicle content efflux. (B) Time course of TMC31-induced release of intravesicular aqueous contents for POPC/PSM/Chol (1) and POPC/PSM/Chol-one (2) 1:7:2 composition. (C) TM31C-induced total release (black bars) and corresponding initial rates (white bars).