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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Dev Biol. 2014 Aug 2;394(1):122–128. doi: 10.1016/j.ydbio.2014.07.018

Figure 2.

Figure 2

The size of the recycled receptor pool at agrin-induced acetylcholine receptor clusters depends on the strength of the agrin stimulus. A) Soleus muscle fibers were either injected with a high dose (1mg/ml) or a low dose (0.1 mg/ml) of plasmids coding for full length chicken agrin, receptor pools (recycled and pre-existing) B) Graph showing that the ratio of recycled/pre-existing AChRs increased with the strength of agrin stimulus. C) Soleus muscles of adult rats were denervated and then injected with either a low or a high concentration of agrin plasmid and a plasmid encoding nls-GFP. Shown are three examples of induced ectopic clusters with a high dose of agrin and one example with a low dose of agrin. D) Graph summarizing the quantification of fluorescence ratios of recycled/pre-existing AChRs at agrin-induced clusters (with a low (N=5) and high dose (N=9) of agrin) and original denervated NMJs (n=6). **P<0.01(One-way ANOVA followed by Tukey’s post test). Note that denervated muscles injected with a high dose of agrin maintained a high level of recycled AChRs at induced ectopic clusters comparable to that at original endplates. Bars, 10 µm for A and 15 µm for B. Bar graph (±s.d).