Fig. 2.

Sirt1 regulates acetylation of autophagy gene products. (a) Transient-increased expression of Sirt1 reduces acetylation. HeLa cells were transfected with the indicated epitope-tagged Atg construct along with, where indicated, wild-type Sirt1 −/−. Measurements were done under fed conditions. IP immunoprecipitation, WB Western blotting. (b) Acetylation of autophagy proteins in wild-type or Sirt1−/− MEFs under fed conditions. Absence of Sirt1 dramatically increased acetylation. (c) Levels of endogenous Atg7 acetylation in various wild-type (+/+) and Sirt1−/− neonatal tissue. Tissues were harvested several hours after birth (reprinted with permission from Lee et al. [6]). (d) Similar analysis in E15.5 embryos