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. Author manuscript; available in PMC: 2014 Sep 18.
Published in final edited form as: Methods Mol Biol. 2013;1077:259–271. doi: 10.1007/978-1-62703-637-5_17

Fig. 3.

Fig. 3

Sirt1 is necessary for autophagy. Transient expression of wild-type (WT) Sirt1 but not a deacetylase-inactive (HY) point mutant of Sirt1 stimulates conversion of LC3-I to LC3-II in HCT116 cells. Quantification of the relative (rel.) levels of LC3-II/LC3-I is shown with the ratio of 1.0 being assigned to vector- transfected cells under fed conditions (reprinted with permission from Lee et al. [6])