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. Author manuscript; available in PMC: 2014 Sep 18.
Published in final edited form as: Methods Mol Biol. 2013;1077:259–271. doi: 10.1007/978-1-62703-637-5_17

Fig. 4.

Fig. 4

Role of Sirt1 in autophagy. (a) Reduced autophagy in Sirt1−/− MEFs. High power view of wild-type or Sirt1−/− MEFs transfected with GFP-LC3 under fed or starved conditions. In wild-type (wt) cells starvation induces an increase in GFP-LC3 punctae. (b) Quantification of GFP-LC3 punctae in wild-type (+/+) and Sirt1−/− MEFs under fed or starved conditions (reprinted with permission from Lee et al. [6])