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. Author manuscript; available in PMC: 2014 Sep 18.
Published in final edited form as: J Clin Neurophysiol. 2010 Dec;27(6):418–424. doi: 10.1097/WNP.0b013e3181fe0709

Figure 6. Spontaneous epileptiform discharges are the result of intrinsic alterations in organotypic slices but requires growth medium.

Figure 6

A. Robust ictal-like discharges in growth medium disappear within ~5 min of washing in standard ACSF. B. 100 min field potential recording from a 7 DIV hippocampal slice culture without GlutaMAX in the growth medium. C. 100 min field potential recording from a 7 DIV hippocampal slice culture with GlutaMAX in the growth medium. D. The threshold between pathological activity and physiological activity (Fig. 1) calculated in 5 min epochs of 100 min recordings with and without GlutaMAX for 7–8 DIV cultures. E. Without the glutamine supplement GlutaMAX in the recording medium, epileptiform activity in 21+ DIV cultures is delayed beyond the standard 60 min recording period.