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. Author manuscript; available in PMC: 2015 Aug 22.
Published in final edited form as: Neuroscience. 2014 May 27;274:153–161. doi: 10.1016/j.neuroscience.2014.05.027

Figure 6. PTEN knockout/inhibition increases GABAA receptor-mediated IPSC and maximal currents.

Figure 6

(A) Representative miniature IPSCs (mIPSCs) in wild type (WT) and Nestin-cre+/PTENloxp/+ (nKO) mice. GABAergic mIPSCs were isolated with 1 mM kynurenic acid and 0.3 μM TTX. The holding potential was -70 mV. (B) Conditional PTEN KO increased amplitude of mIPSCs (WT: n=7; nKO: n=13). (C) Conditional PTEN KO increased GABAA receptor maximal current density (pA/pF) in response to 1 mM GABA recorded in CA1 pyramidal neurons (WT: n=10; nKO: n=9). (D) PTEN inhibitor (BPV 100 nM, 24 hrs) increased maximal GABAA receptor current density activated by 1 mM GABA recorded from single HEK293 cell stably expressing human α1β2γ2 GABAA receptors (n=16 for control (CTL) and BPV).