Fig. 1.

Effect of oAECs, harvested from amnia at different pregnancy times (early vs late), on the PHA-stimulated lymphocyte proliferation. oAECs harvested from early (dark grey bars) or late (light grey bars) pregnancy amnia were used at passage 1 (panels a and c) or passage 3 (panels b and d) of culture and put in contact with PBMNCs (panels a and b) or in a transwell system (panels c and d). oAECs were added to obtain different PBMNC:oAECs ratios (1:1, 1:0.5, 1:0.25, 1:0.125, and 0.0625). Data are represented as mean and SD. The number of the independent experiments performed is indicated inside the bars. ⁺ = p < 0.05, ⁺⁺ = p < 0.01; ⁺⁺⁺ = p < 0.001 versus PHA-stimulated PBMNCs (in absence of oAECs)