Fig. 4.

Expression profile of pluripotency markers in oAECs isolated from early or late gestational amnia. The mRNA content of TERT, NANOG, OCT4A and SOX2 was analyzed in freshly isolated oAECs or in differentiated oAECs towards the adipogenic, osteogenic or neurogenic lineages (a–d) by RT-PCR. The semi-quantitative analyses of mRNA levels were normalized for GAPDH gene and expressed as mean of 3 different replicates ± SD. * significantly different values between freshly and differentiated oAECs within each cell category (p < 0.05); ^a significantly different values between oAECs isolated from early and late gestational age amnia, calculated within a similar category