Fig. 4.

Quadruplex on-chip RPA experiment for the detection of methicilin-resistant Staphylococcus aureus (MRSA), Neisseria gonorrhoeae (NG), Salmonella enterica (SE) and a plasmid (amplification control) (PC) in parallel. Laser scanner images of one subarray (left side) showing clearly visible signals at positions where amplification-specific sequences are immobilized (compare with Fig. 1). Quantitative analysis (right side) exhibit signals well above the dynamic limit of detection (LOD) (horizontal line), while no signals above LOD occur in non-specific or buffer control positions. IC: immobilization control; BC: buffer control; SC: specificity control