. 2014 Feb 18;181(13):1715–1723. doi: 10.1007/s00604-014-1198-5

Table 1.

Primers used in this study

	Target gene	Oligonucleotide sequence [5′-3′]	Amplicon length
Staphylococcus aureus (MRSA)
SA fwd3	mecA	TCCAACATGAAGATGGCTATCGTGTCACAATCGTT	193 bp
SA rev3	mecA	CCTGTTTGAGGGTGGATAGCAGTACCTGAGCC	193 bp
Salmonella enterica
SE fwd1	invA	TACCGGGCATACCATCCAGAGAAAATCGGGCCGC	133 bp
SE rev2	invA	ATTGGCGATAGCCTGGCGGTGGGTTTTGTTGT	133 bp
Neisseria gonorrhoeae
NG fwd3	fit	CAACGCAATCAAATTCCGTGCGCGAGCCGCAG	165 bp
NG rev2	fit	CCGCGTACGTCTTCCAGCTCAACACCTCCGAT	165 bp
Plasmid control
PC fwd5	Plasmid control	ATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGT	227 bp
PC rev6	Plasmid control	CAGCAACGCGGCCTTTTTACGGTTCCTGGCCTT	227 bp

For on-chip RPA experiments forward primers were modified with a 5′ NH₂-(CH₂)₆-linker to allow the covalent attachment to the chip surface. Reverse primers in on-chip RPA experiments contain a 5′ Cy5-modification for signal generation