Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Sep 16;9:23. doi: 10.1186/1749-8546-9-23

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Yang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

RT-PCR assay for induction of follistatin expression. (A) Assays of S. Persicae extract and liquid-liquid extraction products. HepG2 cells were treated with S. Persicae extract at the indicated concentrations and S. Persicae-derived fractions for 24 h, and the expression of follistatin mRNA was detected by RT-PCR. S/N, supernatant; EA, ethyl acetate. (B) HPLC separation of the n-butanol extract. The n-butanol extract was separated by semi-preparative RP-HPLC on a C18 column into 11 fractions. (C) Assays of HPLC fractions. The HPLC fractions were assayed for induction of follistatin mRNA expression.