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. 2014 Sep 16;9:23. doi: 10.1186/1749-8546-9-23

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Copyright © 2014 Yang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Chemical characterization of fraction F5. (A) LC-MS analysis of fraction F5. Positive ESI-MS m/z signals are shown. The chemical structure of amygdalin was generated by ACD/Chem Sketch software (http://www.acdlabs.com/resources/freeware/chemsketch/). (B) HPLC verification of amygdalin as the active compound. The fraction F5, amygdalin standard, and F5-amygdalin mixture were sequentially analyzed by reverse-phase HPLC on a C18 column under the same conditions. (C) RT-PCR verification of commercial amygdalin for induction of follistatin mRNA. HepG2 cells were treated with commercial amygdalin at the indicated concentrations for 24 h. The expression of follistatin mRNA was detected by RT-PCR.