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. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: Theriogenology. 2014 Jul 15;82(7):1043–1048. doi: 10.1016/j.theriogenology.2014.07.010

Figure 1. Trophoblast-specific epifluorescent GFP expression.

Figure 1

Mouse blastocysts (E3.5) devoid of zona pellucida were infected with Lv-CMV-GFP-V5 lentivirus for 4.5 hours and subsequently assessed for GFP expression via epifluorescent microscopy (A). Mouse blastocysts (E3.5) devoid of zona pellucida were infected with Lv-CMV-GFP-V5 and transferred into a pseudo-pregnant female. The feto-placental unit was collected at E10.5 and examined for GFP expression via epifluorescent microscopy (B). E10.5 placentas were sectioned to analyze GFP expression by epifluorescent microscopy in the region that distinguishes the maternal decidua from the developing placenta; (*) parietal trophoblast giant cells, (Dec) maternal uterine decidua, and (Pl) fetal-derived placenta (C); as well as trophoblasts in the placental labyrinth (D); (m) maternal and (f) fetal blood spaces are indicated. An E12.5 feto-placental unit was evaluated for GFP expression by epifluorescent microscopy that demarcates maternal (decidua), placental, and embryonic (embryo) tissue (E). Nuclei (blue) were stained with DAPI.