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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Exp Hematol. 2014 May 20;42(9):761–772.e10. doi: 10.1016/j.exphem.2014.05.005

Figure 6. CD45 reporter marks persistent hematopoiesis following transplantation.

Figure 6

(A): Transplantation scheme showing HSPCs derived from CD45.1 strain being transduced with a CD45 reporter followed by transplantation into irradiated congenic CD45.2 recipients. (B): Peripheral blood stained for CD45.1 to determine chimerism in recipient mice that received CD45.1 HSPCs from mock transduced (n=5) and CD45-GFP (n=5) lentiviral reporter constructs. Error bars represent standard deviation. *** represents a statically significant 2 way ANOVA test with p<0.001. (C): Peripheral blood staining at 20 weeks with antibodies detecting CD3ε (T lymphocytes), Gr-1/Mac-1 (granulocytes and macrophages), Ter119 (erythroid progenitors), and B220 (B lymphocytes). Approximately 50% chimerism resulted following transplantation, and the first panel represents donor (CD45.1) cells. The fraction of GFP positivity for each lineage derived from CD45.1 HSPCs is indicated in the pink square box. The positive population for CD45.1 was further sub-gated to determine the percentage of cells representing myeloid and lymphoid lineages. (D): Same as in (C) except following HSPC transplantation without non-transduced supportive bone marrow. Abbreviation: ANOVA, analysis of variance between groups; HSPC, hematopoietic stem and progenitor cell.