Table 1. List of primers used in this study.
Reporter construction primers (AttB sequences in red, genomic hybridizing sequences in blue) | |
---|---|
CD45 prom −164 AttB1-F | ggggacaagtttgtacaaaaaagcaggcttacctttagaggaaaattgagacga |
CD45 prom +168 AttB2-R | ggggaccactttgtacaagaaagctgggtaaataatggttatctctcttgaagtttg |
Quantitative real-time PCR primers | |
CD34 qRT-PCR-F | ggtagctctctgcctgatgagt |
CD34 qRT-PCR-R | ttggtaggaactgatggggata |
CD45 qRT-PCR-F | ggcagatgatattccaaagaaa |
CD45 qRT-PCR-R | atctccacttccatgtctccat |
Myb qRT-PCR-F | gagatgtgtgaccatgactacga |
Myb qRT-PCR-R | gttctgttccaccagcttcttc |
Vav1 qRT-PCR-F | gcagtgaactctttgaggcttt |
Vav1 qRT-PCR-R | gattcctttgttctgggcaat |
Hprt1 qRT-PCR-F | aactttgctttccctggttaa |
Hprt1 qRT-PCR-R | tcaagggcatatccaacaaca |
Gapdh qRT-PCR-F | tgtgtccgtcgtggatctga |
Gapdh qRT-PCR-R | cctgcttcaccaccttcttga |
Lentiviral vector titering primers | |
LV WPRE-F | accacctgtcagctcctttc |
LV WPRE-R | caacaccacggaattgtcagt |
LV VSV-G-F | ccattattgcccgtcaagctc |
LV VSV-G-R | ggagtgaaggatcggatggact |
LV gag-F | atgggtgcgagagcgtcagt |
LV gag-R | caggccaggattaactgcga |