Figure 1.

Experimental approaches used in the study. (A) Scanning electron micrographs showing the cell morphology before and during spreading. Scale bar, 1.0 μm. Also shown is a schematic of the rough cell surface becoming smooth during spreading and bringing molecules into closer contact with the substrate. (B) Schematic of the chemical coating on the surface. Circles at the surface represent protein G, y-shaped structures are antibodies to His-tag, and the chimeric protein is shown in two shades, light gray for the fractalkine stalk and black for the IL-8 portion. Note that the same chemistry is used on both glass slides and beads. (C) Video micrograph showing the use of micropipettes to bring cells into contact with IL8 immobilized on the bead surface.