Figure 6.

Lateral redistribution of the molecules during neutrophil spreading. (A) A series of images shows the progressive redistribution of L-selectin (middle row) and CXCR1 (lower row) as cells spread onto, then engulf, a glass bead coated with IL8. The upper row shows the brightfield images corresponding to the L-selectin distribution (middle row). LFA-1 (not shown) exhibited behavior similar to that of CXCR1. (B and C) Reconstruction of through-focus fluorescence images of a fixed cell spreading onto an IL8 coverslip. (B) Four of the 29 through-focus images (0.4 μm spacing) used in the 3D reconstructions shown in C. Scale bars, 2.0 μm.