Figure 2.

Temperature dependence of changes in activation free energy ($\Delta \Delta G_i^{\ast }$), activation entropy ($T\Delta \Delta S_i^{\ast }$), and activation enthalpy ($\Delta \Delta H_i^{\ast }$) of control vesicles induced by the presence of VSV-TMD peptide. (A and C) Activation free energy ($\Delta \Delta G_i^{\ast }$) versus temperature for steps 1 and 3, respectively. (B) $T\Delta \Delta S_1^{\ast }$ (solid line) and $\Delta \Delta H_1^{\ast }$ (dotted line) for intermediate formation. (D) $T\Delta \Delta S_3^{\ast }$ (solid line) and $\text{Δ}\text{Δ}{{\rm H}}_3^{\ast }$ (dotted line) for pore formation. Insets show the temperature dependence of changes in activation heat capacity ($\Delta \Delta C{p}_i^{\ast }$) in the presence of the peptide for each step. Coefficients of the polynomials obtained from the raw plots of $\Delta G^{\ast }$ versus T for the vesicles containing TMD are given in Table S2. The plots in B and D were obtained from those in A and C by differentiation.