Table 1.
Kinetic constants for PEG-mediated fusion of PC/PE/SM/CH (35:30:15:20) SUVs containing or not containing VSV-TMD, and in the presence or absence of 2% hexadecane at low and high temperatures
| k1 × 103 (s−1) | k3 × 103 (s−1) | α | β | λ0 × 104 | λ1 × 104 | λ3 × 104 | fCM | (1 − α) × fCM | fLM | |
|---|---|---|---|---|---|---|---|---|---|---|
| 22°C_con | 16.0 ± 0.5 | 1.69 ± 0.01 | 0.18 ± 0.010 | 0.66 ± 0.012 | 2.28 ± 0.07 | 2.09 ± 0.1 | 0.49 ± 0.17 | 0.25 ± 0.01 | 0.21 ± 0.004 | 0.43 ± 0.02 |
| 22°C_VSV | 26.3 ± 0.28 64% | 2.48 ± 0.03 47% | 0.44 ± 0.021 | 0.63 ± 0.015 | 7.87 ± 0.21 | 3.8 ± 0.1 | 0.72 ± 0.16 | 0.26 ± 0.01 | 0.15 ± 0.01 | 0.45 ± 0.01 |
| 22°C_hex | 17.0 ± 0.35 6.25% | 2.21 ± 0.03 31% | 0.57 ± 0.025 | 0.64 ± 0.022 | 7.73 ± 0.06 | 3.49 ± 0.05 | 0.32 ± 0.02 | 0.37 ± 0.01 | 0.16 ± 0.02 | 0.44 ± 0.02 |
| 22°C_hex + VSV | 26.0 ± 0.46 0% | 2.79 ± 0.04 12% | 0.60 ± 0.01 | 0.64 ± 0.01 | 18.3 ± 0.02 | 4.8 ± 0.1 | 0.5 ± 0.2 | 0.36 ± 0.02 | 0.14 ± 0.02 | 0.43 ± 0.02 |
| 37°C_con | 66.8 ± 1.5 | 3.03 ± 0.04 | 0.36 ± 0.021 | 0.72 ± 0.03 | 40.28 ± 0.35 | 7.24 ± 0.07 | 8.99 ± 0.28 | 0.33 ± 0.01 | 0.21 ± 0.01 | 0.46 ± 0.02 |
| 37°C_VSV | 117.9 ± 2.0 76% | 3.36 ± 0.03 11% | 0.56 ± 0.011 | 0.77 ± 0.04 | 51.9± 0.5 | 8.62 ± 0.07 | 11.50 ± 0.38 | 0.34 ± 0.02 | 0.15 ± 0.02 | 0.47 ± 0.03 |
| 37°C_hex | 89.0 ± 2.3 33% | 3.83 ± 0.15 26% | 0.68 ± 0.023 | 0.75 ± 0.03 | 87.87 ± 0.31 | 7.87 ± 0.36 | 4.96 ± 0.45 | 0.43 ± 0.03 | 0.14 ± 0.01 | 0.46 ± 0.02 |
| 37°C_hex + VSV | 95.9 ± 1.6 18.7% | 3.64 ± 0.11 0% | 0.66 ± 0.02 | 0.74 ± 0.03 | 83.9 ± 0.2 | 9.1 ± 0.2 | 8.6 ± 0.5 | 0.42 ± 0.01 | 0.14 ± 0.02 | 0.47 ± 0.03 |
The percentages beside the k1 and k3 values for VSV-TMD and hexadecane alone are changes relative to control, and the percentages below VSV-TMD + hexadecane (shown in bold italics) are relative to control + VSV-TMD.